Research

Research topics

Enzymology. Enzyme kinetics. Transient phase and steady-state. Rapid equilibrium.

Analytical and numerical resolution of mechanisms. Mathematical modellization. Computer simulation.

Enzyme extraction. Differential centrifugation.

Mechanism of Polyphenol oxidase (EC 1.14.18.1). Mono- and diphenolase activities. Effect of inhibitors. Latency and kinetic cooperativity. Enzymatic synthesis of o-diphenols.

Enzymatic inhibition (classical inhibitors, time dependent inhibitors, unstable inhibitors, tight-binding inhibitors).

Mechanisms of enzymatically catalyzed reactions involving one or more unstable species.

Zymogen activation processes. Kinetics of the reaction in the presence of a chromogenic substrate. Effect of different inhibitors. Pepsinogen, plasminogen, trypsinogen, etc.

Biological systems of cascade amplification.

Substrate cycles. Analytical and clinical applications. Economical optimization. Amplification of an enzymatic activity.

Glutathione-ascorbate redox cycle in chloroplasts. Chemical and enzymatic reactions involved. Oxidative stress.

Oxidoreductase enzymes: Polyphenol oxidase, Peroxidase and Laccase.

Residence time of an enzymatic system.

 Research grants: Founded projects as main researcher

Kinetic study of biocatalytic processes using oxidoreductase enzymes. Founded by Junta de Comunidades de Castilla-La Mancha, PAI08-0175-8618. 2008-2010.

Kinetic analysis of several biological mechanisms of response amplification against a signal. Founded by Junta de Comunidades de Castilla-La Mancha, PAI05-036. 2005-2007.